How to add sample for Food Safety ELISA kit
1. Add Standard and Sample into micro-wells as shown in the following sheet, 50ul/well:
1-1 Single-well test
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12
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A
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Standard 1
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Sample 3
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B
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Standard 2
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Sample 4
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C
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Standard 3
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Sample 5
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D
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Standard 4
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Sample 6
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E
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Standard 5
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Sample 7
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F
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Standard 6
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Sample 8
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G
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Sample 1
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Sample 9
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H
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Sample 2
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Sample 10
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1-2 Double-well test
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2
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3
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4
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10
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11
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12
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A
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Standard 1
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Standard 5
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Sample 3
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Sample 7
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B
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Standard 1
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Standard 5
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Sample 3
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Sample 7
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C
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Standard 2
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Standard 6
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Sample 4
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Sample 8
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D
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Standard 2
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Standard 6
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Sample 4
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Sample 8
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E
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Standard 3
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Sample 1
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Sample 5
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Sample 9
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F
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Standard 3
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Sample 1
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Sample 5
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Sample 9
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G
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Standard 4
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Sample 2
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Sample 6
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Sample 10
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H
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Standard 4
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Sample 2
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Sample 6
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Sample 10
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2. Add Enzyme Conjugate, 50ul/well into micro-wells already add standard and sample
3. Add Antibody working solution, 50ul/well into micro-wells already add standard/sample and Enzyme conjugate, cover and shake gently, incubate at 25℃ for 30min.
Shenzhen Lvshiyuan Biotechnology Co., Ltd